ITGA6 antibody

Catalog No. ABIN201115

This Mouse Monoclonal antibody specifically detects ITGA6 in WB, IHC, IP and ICC. It exhibits reactivity toward Chicken.

Quick Overview for ITGA6 antibody (ABIN201115)

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-ITGA6 Antibody

Specificity: Reactive with chicken integrin alpha6, as demonstrated by experiments with cDNA-transfected NIH3T3 cells followed by immunoblots.

Purification: Purified

Immunogen: Integrin alpha subunits purified from adult chicken brain tissue, denatured in 6 M guanidine isothiocyanate, and depleted of beta1 subunit.

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IP, WB

Usage: Western Blot. Immunoprecipitation: Precipitates the monomeric alpha6 subunit after the dimeric receptor has been denatured, but does not precipitate the native receptor. Immunohistochemistry: Methanol-fixed cryosections. An overnight methanol fix is suggested. Previous experiments have used 14 micron sections of chicken embryonic tissue. Typical antibodies dilutions are 35 μg/mL, with overnight antibody incubation, followed by detection with a goat anti-mouse IgG-FITC secondary antibody. Immunocytochemistry: Methanol-fixed cultured cells (15 minute fixation). Western Blot: Recognizes a 130kD band in chicken brain tissue and a 140kD band in transfected NIH3T3 cells (the difference in molecular weight is likely due to differential glycosylation).

Comment:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for ITGA6

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Alternative Name: ITGA6/Integrin Alpha 6/CD49f

Background: Name/Gene ID: ITGA6
Family: Integrin

Synonyms: ITGA6, CD49f, CD49f antigen, Integrin alpha6B, Integrin, alpha 6, Integrin alpha-6 precursor, VLA-6, Integrin alpha-6, ITGA6B

Gene ID: 3655

UniProt: P23229

Pathways: CXCR4-mediated Signaling Events, Brown Fat Cell Differentiation, Integrin Complex