MLANA antibody

Catalog No. ABIN201283

This Mouse Monoclonal antibody specifically detects MLANA in WB, IHC, IHC (p), IP and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for MLANA antibody (ABIN201283)

Target: MLANA (Melan A (MLANA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLANA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MLANA Antibody

Specificity: Recognizes MART-1 at ~18kD. Does not cross-react with MAGE-1 or tyrosinase protein. Species Reactivity: Human.

Purification: Protein G purified

Immunogen: Recombinant MART-1 protein.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-Fr, IHC-P, IP, WB (1 μg/mL)

Usage: Suitable for use in Immunoprecipitation, Western Blot, and Immunohistochemistry (frozen & formalin/paraffin). Immunoprecipitation: Use Protein G, 2 μg antibody/mg protein lysate. Western Blot: 1 μg/mL for 2 hours at RT. Immunohistochemistry: 1-2 μg/mL for 30 min. at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0 for 10-20 min., followed by cooling at RT for 20 min. Positive control: CaC1 melanoma cells and melanomas. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4.

Preservative: Azide free

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MLANA

Target: MLANA (Melan A (MLANA))

Alternative Name: MLANA / Melan-A / MART-1

Background: Name/Gene ID: MLANA

Synonyms: MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A, Protein Melan-A, MART-1, MART1

Gene ID: 2315

UniProt: Q16655