MLANA antibody

Catalog No. ABIN201284

The Mouse Monoclonal anti-MLANA antibody has been validated for WB, IHC, IHC (p), IP and IHC (fro). It is suitable to detect MLANA in samples from Human and Horse.

Quick Overview for MLANA antibody (ABIN201284)

Target: MLANA (Melan A (MLANA))

Reactivity: Human, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLANA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MLANA Antibody

Specificity: Recognizes MART-1 at 18kD. Species cross-reactivity: Human and equine.

Purification: Protein A purified

Immunogen: Recombinant hMART-1 protein.

Type of Immunogen: Recombinant protein

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: IHC, IHC-Fr (2 - 4 μg/mL), IHC-P (2 - 4 μg/mL), IP, WB (0.5 μg/mL)

Usage: Immunoprecipitation, Western Blot, Immunohistochemistry (frozen & formalin/paraffin). Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0 for 10-20 min., followed by cooling at RT for 20 min. Immunoprecipitation: Use Protein A, 2 μg antibody/mg protein lysate. Western Blot: 0.5 μg/mL. Immunohistochemistry: 2-4 μg/mL. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4.

Preservative: Azide free

Target Details for MLANA

Target: MLANA (Melan A (MLANA))

Alternative Name: MLANA / Melan-A / MART-1

Background: Name/Gene ID: MLANA

Synonyms: MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A, Protein Melan-A, MART-1, MART1

Gene ID: 2315

UniProt: Q16655