SPARC antibody

Catalog No. ABIN201350

The Mouse Monoclonal anti-SPARC antibody has been validated for WB, IHC, ELISA and FACS. It is suitable to detect SPARC in samples from Human and Cow.

Quick Overview for SPARC antibody (ABIN201350)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: ON1-1

Product Details anti-SPARC Antibody

Specificity: Recognizes bovine Osteonectin. Specific for bone-derived and platelet-derived proteins. Reacts with thrombin-stimulated platelet in Flow Cytometry. Does not react with non-activated platelet. Species cross-reactivity: human.

Purification: Ion exchange chromatography

Immunogen: Bovine Osteonectin.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (1:4000), Flo (1 μg/mL), IHC (5 - 10 μg/mL), WB (5 - 10 μg/mL)

Usage: Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Flow Cytometry: 1 μg/mL. ELISA: 1:4000 (0.5 μg/mL). Western Blot: 5-10 μg/mL, Reducing or non-reducing conditions, EDTA-extracted fraction of bovine bone. Immunohistochemistry: 5-10 μg/mL (human cartilage tissue).

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile 40-50 % glycerol, ddH2O

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 1 % BSA, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Lyophilized and resuspended forms: store at -20°C.

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: Osteonectin / SPARC

Background: Name/Gene ID: SPARC

Synonyms: SPARC, Basement-membrane protein 40, BM-40, Cysteine-rich protein, ON, Osteonectin

Gene ID: 6678

UniProt: P09486

Pathways: Autophagy