TJP2 antibody

Catalog No. ABIN201479

The Rabbit Polyclonal anti-TJP2 antibody has been validated for WB, ELISA and IHC. It is suitable to detect TJP2 in samples from Human, Mouse and Dog.

Quick Overview for TJP2 antibody (ABIN201479)

Target: TJP2 (Tight Junction Protein 2 (Zona Occludens 2) (TJP2))

Reactivity: Human, Mouse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TJP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-TJP2 Antibody

Specificity: Specific for the canine ZO-2 protein (~160kD). Does exhibit cross-reactivity with a few proteins of unknown identity in the 50-85kD region. Does not appear to cross-react with the related ZO-1 protein. Species cross-reactivity: human and mouse.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the central portion of the ZO-2 protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC, WB (1 - 4 μg/mL)

Usage: Suitable for use in ELISA, Immunohistochemistry and Western Blot. ELISA: 0.1-1 μg/mL. Western Blot: 1-4 μg/mL. Immunohistochemistry (Mouse liver tissues): 4-5 μg/mL. Note: Weak fixation of tissues with 2 % paraformaldehyde/picric acid followed by NO post-fixation is required to produce staining. Positive control: HeLa and canine MDCK cell lysates and mouse liver homogenates.

Comment:

Target Species of Antibody: Dog

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for TJP2

Target: TJP2 (Tight Junction Protein 2 (Zona Occludens 2) (TJP2))

Alternative Name: TJP2 / ZO2 / ZO-2

Background: Name/Gene ID: TJP2
Family: Occludin

Synonyms: TJP2, C9DUPq21.11, DUP9q21.11, DFNA51, ZO-2, Zona occludens 2, Zonula occludens protein 2, Tight junction protein 2, Tight junction protein ZO-2, ZO2, X104, Zona occludens protein 2

Gene ID: 9414

UniProt: Q9UDY2