Cadherin 13 antibody (AA 23-692)

Catalog No. ABIN203820

This anti-Cadherin 13 antibody is a Rat Monoclonal antibody detecting Cadherin 13 in WB, ELISA and FACS. Suitable for Human.

Quick Overview for Cadherin 13 antibody (AA 23-692) (ABIN203820)

Target: Cadherin 13 (CDH13)

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This Cadherin 13 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Clone: 392411

Product Details anti-Cadherin 13 Antibody

Binding Specificity: AA 23-692

Specificity: Recognizes human Cadherin-13. Does not cross-react with rhECAD, rhNCAD, rhPCAD, rhVECAD, rhCAD-8, rhCAD-11, rhCAD-12 or rhCAD-17.

Purification: Protein G purified

Immunogen: NS0-derived recombinant human Cadherin-13 (aa23-692) Accession # P55290.

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Flow Cytometry and ELISA. Direct ELISA: 0.5-1 μg/mL, detection limit: 7 ng/well. Western Blot: 1-2 μg/mL, detects 5 ng/lane under non-reducing conditions. Not recommended for use under reducing conditions. Flow Cytometry: 25 μg/mL. Add 10 μL of the diluted. solution to 1-2.5 x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Positive control: NCI-H460 human large cell lung cancer cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS with 5 % trehalose

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Cadherin 13

Target: Cadherin 13 (CDH13)

Alternative Name: CDH13 / Cadherin 13

Background: Name/Gene ID: CDH13
Family: Cadherin

Synonyms: CDH13, Cadherin-13, CDHH, Cdht, Heart cadherin, H-cadherin, T-cad, T-cadherin, Truncated cadherin, p105

Gene ID: 1012

UniProt: P55290

Pathways: EGFR Signaling Pathway, Cell-Cell Junction Organization