CD28 antibody

Catalog No. ABIN203924

The Mouse Monoclonal anti-CD28 antibody is suitable to detect CD28 in samples from Human. It has been validated for FACS, IP, IHC and IHC (fro).

Quick Overview for CD28 antibody (ABIN203924)

Target: CD28

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CD28 Antibody

Specificity: Recognizes CD28 expressed on T cells and also on BHK cells transfected with human CD28. Monomeric and cross-linked antibody will stimulate expression of IL-2 from CD28+ cells.

Purification: Protein A purified

Immunogen: Human CD28 Ig fusion protein and HPB-MLT human tumor cells.

Type of Immunogen: Fusion protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: Flo (5 μg/mL), IHC, IHC-Fr, IP

Usage: Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry. Immunohistochemistry: Frozen sections. Flow Cytometry: 5 μg/mL labels 5x10^5 cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.5 mg/mL Gentamicin sulfate.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: Name/Gene ID: CD28

Synonyms: CD28, CD28 antigen, CD28 antigen (Tp44), Tp44, CD28 Molecule

Gene ID: 940

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response