Chemerin antibody

Catalog No. ABIN204116

The Rat Monoclonal anti-Chemerin antibody has been validated for WB, ELISA and Neut. It is suitable to detect Chemerin in samples from Mouse.

Quick Overview for Chemerin antibody (ABIN204116)

Target: Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Chemerin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-Chemerin Antibody

Specificity: Selected for its ability to detect mouse Chemerin in direct ELISAs and Western Blots.

Purification: Protein G purified

Immunogen: E. coli-derived rmChemerin.

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Neut (1 - 5 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Neutralization and ELISA. Western Blot: 1-2 μg/mL. Using a colorimetric detection system, the detection limit is ~10 ng/lane under non-reducing conditions. Chemiluminescent detection will increase sensitivity 5-50 fold. Neutralization: 1-5 μg/mL in the presence of 100 ng/mL of rmChemerin, using the human ChemR23 transfected BaF/3 cell chemotaxis assay. ELISA: 0.5-1.0 μg/mL. Detection limit is ~10 ng/well. Hybridoma: Mouse myeloma with B cells obtained from a rat immunized with purified, E. coli-derived, recombinant mouse Chemerin (rmChemerin, aa17-156, Accession # NP_082128).

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Handling Advice: Aviod frequent freeze thaws.

Storage: -20 °C

Storage Comment: Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.

Target Details for Chemerin

Target: Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))

Alternative Name: RARRES2 / Chemerin

Background: Name/Gene ID: RARRES2

Synonyms: RARRES2, HP10433, RAR-responsive protein TIG2, Tazarotene-induced gene 2, TIG2, Chemerin

Gene ID: 5919

UniProt: Q99969

Pathways: Brown Fat Cell Differentiation