DLL1 antibody (Extracellular Domain)

Catalog No. ABIN204181

This Mouse Monoclonal antibody specifically detects DLL1 in ELISA, IHC, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for DLL1 antibody (Extracellular Domain) (ABIN204181)

Target: DLL1 (delta-Like 1 (DLL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DLL1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DLL1 Antibody

Binding Specificity: AA 22-540, Extracellular Domain

Specificity: Selected for its ability to detect human DLL1 in direct ELISAs. Does not cross-react with rhDLL3 or rhDLL4.

Purification: Protein G purified

Immunogen: Recombinant corresponding to aa22-540 from human Delta-like 1 expressed in NSO cells (Accession #AAG09716).

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), ICC, IHC, IHC-P

Usage: Suitable for use in ELISA, Immunocytochemistry and Immunohistochemistry. Between each step, cells were washed with PBS containing 0.1 % BSA. Immunohistochemistry: 25 μg/mL to detect DLL1 in paraffin-embedded human squamous cell carcinoma tissue sections. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human DLL1. The detection limit for rhDLL1 is ~6 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized, PBS, 5 % trehalose

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for DLL1

Target: DLL1 (delta-Like 1 (DLL1))

Alternative Name: DLL1

Background: Name/Gene ID: DLL1

Synonyms: DLL1, Delta-like 1 (Drosophila), Delta (Drosophila)-like 1, Drosophila Delta homolog 1, Delta-like protein 1, Delta1, H-Delta-1, DL1, Npa

Gene ID: 28514

UniProt: O00548

Pathways: Notch Signaling, Stem Cell Maintenance