NCK1 antibody (AA 1-254)

Catalog No. ABIN204421

This Rabbit Polyclonal antibody specifically detects NCK1 in WB and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NCK1 antibody (AA 1-254) (ABIN204421)

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-NCK1 Antibody

Binding Specificity: AA 1-254

Specificity: Recognizes a human 47kD SH2/SH3-containing protein, Nck. Species cross-reactivity: Mouse and rat.

Purification: Protein G purified

Immunogen: Purified GST fusion protein corresponding to amino acids 1 to 254 of human Nck, produced in E. coli.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: IP, WB (0.1 - 1 μg/mL)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using goat anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5 μg immunoprecipitates Nck from a lysate of human A431 carcinoma cells, mouse 3T3/A31 fibroblasts and rat L6 skeletal fibroblasts.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for NCK1

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Alternative Name: NCK1 / NCK

Background: Name/Gene ID: NCK1

Synonyms: NCK1, Cytoplasmic protein nck, Cytoplasmic protein NCK1, NCK adaptor protein 1, Nck-1, NCKalpha, Melanoma NCK protein, NCK, NCK tyrosine kinase

Gene ID: 4690

UniProt: P16333

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals