TFPI antibody

Catalog No. ABIN204829

This Mouse Monoclonal antibody specifically detects TFPI in WB, IP and ELISA. It exhibits reactivity toward Human.

Quick Overview for TFPI antibody (ABIN204829)

Target: TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TFPI antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Product Details anti-TFPI Antibody

Specificity: Detects human TFPI in direct ELISAs and Western Blots. Shows no cross-reactivity with rmTFPI or rhTFPI-2.

Purification: Protein G purified

Immunogen: NS0-derived rhTFPI.

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, IP, WB

Usage: Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human TFPI. Using a colorimetric detection system, the detection limit for rhTFPI is approximately 1 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human TFPI. The detection limit for rhTFPI is approximately 2 ng/well. Immunoprecipitation: This antibody was used at a concentration of 25 μg/mL to immunoprecipitate rhTFPI from conditioned cell culture medium. The recovered rhTFPI can be detected in Western blots using a polyclonal antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for TFPI

Target: TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))

Alternative Name: TFPI /LACI

Background: Name/Gene ID: TFPI

Synonyms: TFPI, Anti-convertin, Extrinsic pathway inhibitor, EPI, LACI, TFI, TFPI1

Gene ID: 7035

UniProt: P10646