TFPI antibody

Catalog No. ABIN204830

The Rat Monoclonal anti-TFPI antibody has been validated for WB and ELISA. It is suitable to detect TFPI in samples from Mouse.

Quick Overview for TFPI antibody (ABIN204830)

Target: TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TFPI antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TFPI Antibody

Specificity: Recognizes mouse rmTFPI in direct ELISAs and Western Blots. Shows no cross-reactivity with rhTFPI or rmTFPI-2. Species sequence homology: rat, 73 % , human/chimpanzee, 61 %, and canine 54 %.

Purification: Protein G purified

Immunogen: NS0-derived rmTFPI.

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot and ELISA. Western Blot:1-2 μg/mL with the appropriate secondary reagents to detect mouse TFPI. Using a colorimetric detection system, the detection limit is ~10 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse TFPI. The detection limit is ~1 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 40-50 % glycerol, PBS.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details for TFPI

Target: TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))

Alternative Name: TFPI /LACI

Background: Name/Gene ID: TFPI

Synonyms: TFPI, Anti-convertin, Extrinsic pathway inhibitor, EPI, LACI, TFI, TFPI1

Gene ID: 7035

UniProt: P10646