Vitronectin antibody

Catalog No. ABIN204930

This anti-Vitronectin antibody is a Mouse Monoclonal antibody detecting Vitronectin in WB, ELISA, IHC and IHC (fro). Suitable for Cow and Sheep.

Quick Overview for Vitronectin antibody (ABIN204930)

Target: Vitronectin (VTN)

Reactivity: Cow, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vitronectin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Vitronectin Antibody

Specificity: Highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). Cross-reacts with sheep vitronectin but not with human or horse vitronectin. Suitable for ELISA, immunoblotting and immunostaining of frozen PLP-fixed sections of bovine tissues. The antibody can be used as an affinity purification reagent of vitronectin from bovine plasma or serum and to quantitatively deplete plasma or serum of vitronectin. It can also be used to probe vitronectin conformation.

Purification: Protein A/G purified

Immunogen: Lysed bovine corneal endothelial cells and extracellular matrix.

Type of Immunogen: Cells

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (1:80000), IHC, IHC-Fr, WB (1:200)

Usage: Suitable for use in ELISA, Western Blot, and I immunohistochemistry. The antibody can be used as an affinity purification reagent of vitronectin from bovine plasma or serum and to quantitatively deplete plasma or serum of vitronectin. It can also be used to probe vitronectin conformation. ELISA: 1:80000 (ref. 1,2,3). Western Blot: 1:200. Immunohistochemistry: 1:50 using frozen, PLP-fixed sections of bovine tissues.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 15 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for Vitronectin

Target: Vitronectin (VTN)

Alternative Name: VTN / Vitronectin

Background: Name/Gene ID: VTN
Family: Matrix Protein

Synonyms: VTN, Epibolin, Serum-spreading factor, VNT, S-protein, Somatomedin B, V75, VN, Complement S-protein, Serum spreading factor, Vitronectin

Gene ID: 7448

UniProt: P04004

Pathways: Autophagy, Smooth Muscle Cell Migration