Nestin antibody

Catalog No. ABIN205661

The Mouse Monoclonal anti-Nestin antibody has been validated for WB, IHC and IHC (fro). It is suitable to detect Nestin in samples from Mouse and Rat.

Quick Overview for Nestin antibody (ABIN205661)

Target: Nestin (NES)

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: Rat-401-4D4

Product Details anti-Nestin Antibody

Specificity: Recognizes rat nestin, a large intermediate filament protein transiently expressed in embryonic glial cells. It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas.

No Cross-Reactivity: Human

Purification: Purified

Immunogen: Nestin purified from embryonic rat spinal cord.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-Fr (1:20 - 1:200), WB

Usage: Immunohistochemistry: We recommend perfusing tissues with 4 % paraformaldehyde at  pH 7.4 for light microscopy or with either 4 % paraformaldehyde at  pH 10.0 or 4 % paraformaldehyde with 0.1 % glutaraldehyde at  pH 7.4 for EM. For Immunocytochemistry we recommend using cells fixed in 4 % paraformaldehyde buffered with 50 mM sodium borate at  pH 9.5. Western Blot: LS-C42054 reacts with a band at 200-220 kD in reducing gels of newborn rat or mouse cell extracts. For the purpose of western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris,  pH 6.8, 10 % 2-mercaptoethanol, 10 % glycerol and 4.6 % SDS. Membranes should be blocked with milk or BSA. 5 % PAGE gels are suggested.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin

Background: Name/Gene ID: NES

Synonyms: NES, Nestin, Nbla00170

Gene ID: 10763