BMP4 antibody

Catalog No. ABIN205672

This Mouse Monoclonal antibody specifically detects BMP4 in WB, ELISA and ICC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for BMP4 antibody (ABIN205672)

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This BMP4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Clone: 3H2-3

Product Details anti-BMP4 Antibody

Specificity: Recognizes mouse BMP4. Recognizes human and mouse BMP4 in both reduced and non-reduced conditions. Does not cross-react with human BMP2 or human TGF-b1. Species cross-reactivity: human and rat.

Purification: Protein A purified

Immunogen: Recombinant mouse BMP4.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA, ICC, WB

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Western Blot (Reduced and Non-reduced): Using osteoinductive cells, i. e. BFP and Saos-2. Immunocytochemistry: Using osteoinductive cells, i. e. BFP and Saos-2. Immunohistochemistry (paraffin): 1:2.5 with autoclave/citrate buffer antigen retrieval. Demonstrates the presence of BMP4 in developmental bone formation in human embryo growth plate and the alveolar bone of rat embryo. Positive control: Mouse brain extract.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for BMP4

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Alternative Name: BMP4 / BMP-4

Background: Name/Gene ID: BMP4
Family: TGF beta

Synonyms: BMP4, BMP-2B, BMP-4, BMP2B, BMP2B1, Bone morphogenetic protein 2B, Bone morphogenetic protein 4, DVR4, MCOPS6, OFC11, Zyme

Gene ID: 652

UniProt: P12644

Pathways: Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development