EGF antibody (Extracellular Domain)

Catalog No. ABIN205919

The Rabbit Polyclonal anti-EGF antibody has been validated for WB, IHC, ELISA, IHC (p) and Func. It is suitable to detect EGF in samples from Human.

Quick Overview for EGF antibody (Extracellular Domain) (ABIN205919)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Product Details anti-EGF Antibody

Binding Specificity: Extracellular Domain

Specificity: Recognizes human Epidermal Growth Factor, a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, expressed by a variety of cells. The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the ~170 kD EGF receptor (EGFR), which also binds other EGF-like ligands with high affinity, including TGF-a (transforming growth factor alpha), VGF (vaccinia virus growth factor) and Betacellulin, expressed in the pancreas, small intestine and by certain tumor cells. Receptor activation through EGF binding triggers several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways.

Purification: Purified

Immunogen: Recombinant human EGF.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Func (0.25 - 0.35 μg/mL), IHC, IHC-P (0.25 - 1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This product requires protein digestion pre-treatment of paraffin sections e. g. trypsin or pronase. Histology Positive Control Tissue: Human skin ELISA: LS-C42950 may be used in an indirect ELISA system or as a capture antibody in a sandwich ELISA for human EGF in combination with LS-C42951 as a detection reagent.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water. For long term storage the addition of 0.09 % sodium azide is recommended. For functional studies do not add azide.

Concentration: Lot specific

Buffer: Lyophilized, PBS,  pH 7.4.

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF

Background: Name/Gene ID: EGF

Synonyms: EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor

Gene ID: 1950

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation