EGFR antibody (Extracellular Domain) (FITC)

Catalog No. ABIN206346

This anti-EGFR antibody is a Rat Monoclonal antibody detecting EGFR in FACS. Suitable for Human.

Quick Overview for EGFR antibody (Extracellular Domain) (FITC) (ABIN206346)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This EGFR antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Product Details anti-EGFR Antibody (FITC)

Binding Specificity: Extracellular Domain

Specificity: Recognizes the human epidermal growth factor receptor (EGF-R), which is over expressed in a high proportion of breast cancer cells and in a range of other carcinomas. High level expression of EGFR is often associated with advanced disease and poor prognosis. Binds to epitope B from EGFR and has an affinity of 6.7 x 10-9 M.

Purification: Ion exchange chromatography

Immunogen: Extracellular domain of human EGF-receptor from head and neck carcinoma.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:10)

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is photosensitive and should be protected from light. Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR

Background: Name/Gene ID: EGFR
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: EGFR, EGFf receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1

Gene ID: 1956

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins