MADCAM1 antibody (Biotin)

Catalog No. ABIN206532

This anti-MADCAM1 antibody is a Mouse Monoclonal antibody detecting MADCAM1 in ELISA and FACS. Suitable for Human.

Quick Overview for MADCAM1 antibody (Biotin) (ABIN206532)

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MADCAM1 antibody is conjugated to Biotin

Application: ELISA, Flow Cytometry (FACS)

Clone: 314G8

Product Details anti-MADCAM1 Antibody (Biotin)

Specificity: Recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1), a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognize MAdCAM-1 in these tissues. Clone Reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7. XXX recommend the use of MCA2320EL for this purpose.

Purification: Protein G purified

Immunogen: Recombinant soluble MAdCAM-1-fc fusion protein.

Type of Immunogen: Fusion protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (1 - 10 μg/mL), Flo (1:1 - 1:10)

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for MADCAM1

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Alternative Name: MAdCAM-1

Background: Name/Gene ID: MADCAM1

Synonyms: MADCAM1, HMAdCAM-1, MACAM1, MAdCAM-1

Gene ID: 8174