ITGA6 antibody (PE)

Catalog No. ABIN206771

The Rat Monoclonal anti-ITGA6 antibody has been validated for FACS, IHC, IP and IHC (fro). It is suitable to detect ITGA6 in samples from Human, Mouse, Dog, Pig, Sheep and Cynomolgus.

Quick Overview for ITGA6 antibody (PE) (ABIN206771)

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Reactivity: Human, Mouse, Dog, Pig, Sheep, Cynomolgus

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA6 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ITGA6 Antibody (PE)

Specificity: Recognizes CD49F (VLA-6 alpha chain), a 120kD cell surface glycoprotein that can form distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells. Clone Reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukemia cells (alpha-6 beta-1). In immunohistology the monoclonal Reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts. VLA-6 is an important mediator of cell binding to laminin. Studies suggest that clone GoH3 specifically blocks the binding of cells to the E8 fragment of laminin. XXX recommend the use of MCA699XZ for this purpose.

Purification: Protein G purified

Immunogen: BALB/c mouse mammary tumor cells.

Type of Immunogen: Cells

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:1 - 1:5), IHC, IHC-Fr, IP

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 platelets in 100 μL. Method sheets are available on request.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized powder from PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA, 5 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details for ITGA6

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Alternative Name: ITGA6/Integrin Alpha 6/CD49f

Background: Name/Gene ID: ITGA6
Family: Integrin

Synonyms: ITGA6, CD49f, CD49f antigen, Integrin alpha6B, Integrin, alpha 6, Integrin alpha-6 precursor, VLA-6, Integrin alpha-6, ITGA6B

Gene ID: 3655

UniProt: P23229

Pathways: CXCR4-mediated Signaling Events, Brown Fat Cell Differentiation, Integrin Complex