CD16 antibody

Catalog No. ABIN206894

The Mouse Monoclonal anti-CD16 antibody is suitable to detect CD16 in samples from Human. It has been validated for FACS, IP, WB, Func, BR and Neut.

Quick Overview for CD16 antibody (ABIN206894)

Target: CD16

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Functional Studies (Func), Blocking Reagent (BR), Neutralization (Neut)

Clone: MEM-154

Product Details anti-CD16 Antibody

Specificity: Reacts with the epitope on CD16 antigen that residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen). Recognizes a polymorphism of CD16a (NK-cells form), it reacts well with granulocyte form (CD16b) of all subjects

Purification: Protein A purified

Immunogen: Human granulocytes.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Block, Flo (5 - 10 μg/mL), Func, IP (5 - 10 μg/mL), Neut, WB (5 - 10 μg/mL)

Usage: Flow Cytometry: Recommended dilution - 5-10 μg/mL. Positive control - PBL (peripheral blood lymphocytes). Application note - The antibody MEM-154 does not react with CD16a present on NK cells in many subjects. Western Blotting: Application note - Non-redu The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Azide free PBS, preserved by filter sterilization, approx,  pH 7.4.

Preservative: Azide free

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CD16

Target: CD16

Alternative Name: CD16

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process