CD16 antibody

Catalog No. ABIN206950

This Mouse Monoclonal antibody specifically detects CD16 in FACS, IP, WB, Func, BR and Neut. It exhibits reactivity toward Human.

Quick Overview for CD16 antibody (ABIN206950)

Target: CD16

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Functional Studies (Func), Blocking Reagent (BR), Neutralization (Neut)

Clone: MEM-154

Product Details anti-CD16 Antibody

Specificity: Reacts with the epitope on CD16 antigen that residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen). Recognizes a polymorphism of CD16a (NK-cells form), it reacts well with granulocyte form (CD16b) of all subjects

Purification: Protein A purified

Immunogen: Human granulocytes.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Block, Flo (5 - 10 μg/mL), Func, IP (5 - 10 μg/mL), Neut, WB (5 - 10 μg/mL)

Usage: Flow Cytometry: Recommended dilution - 5-10 μg/mL. Positive control - PBL (peripheral blood lymphocytes). Application note - The antibody MEM-154 does not react with CD16a present on NK cells in many subjects. Western Blotting: Application note - Non-redu

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris buffered saline (TBS), 15 mM sodium azide, approx,  pH 8.0.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CD16

Target: CD16

Alternative Name: CD16

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process