CD53 antibody

Catalog No. ABIN206962

This Mouse Monoclonal antibody specifically detects CD53 in FACS, WB, IHC, IP, Func and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for CD53 antibody (ABIN206962)

Target: CD53

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD53 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MEM-53

Product Details anti-CD53 Antibody

Specificity: Reacts with CD53, a 32-40 kD tetraspanin family glycoprotein exclusively expressed on leukocytes, it is not present on platelets, red blood cells and non-hematopoietic cells. Reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kD using endoglycosidase F).

Purification: Protein A purified

Immunogen: Leukocytes of patient suffering from a LGL-type leukemia.

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, Func, IHC, IHC-Fr, IP, WB

Usage: Flow Cytometry: Recommended dilution - 4 μg/mL. Immunohistochemistry (frozen sections): It is suitable for discrimination of lymphomas from other tumors. Functional Application: The antibody MEM-53 induces activation of monocytes and B lymphocytes.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CD53

Target: CD53

Alternative Name: CD53

Background: Name/Gene ID: CD53
Family: Tetraspan

Synonyms: CD53, CD53 antigen, CD53 Molecule, CD53 tetraspan antigen, Cell surface antigen, Leukocyte surface antigen CD53, Transmembrane glycoprotein, Tetraspanin-25, Tspan-25, CD53 glycoprotein, Cell surface glycoprotein CD53, MOX44, TSPAN25

Gene ID: 963

UniProt: P19397