CD53 antibody

Catalog No. ABIN206962

Product Details anti-CD53 Antibody

Target: CD53

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD53 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Reacts with CD53, a 32-40 kD tetraspanin family glycoprotein exclusively expressed on leukocytes, it is not present on platelets, red blood cells and non-hematopoietic cells. Reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kD using endoglycosidase F).

Purification: Protein A purified

Immunogen: Leukocytes of patient suffering from a LGL-type leukemia.

Clone: MEM-53

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, Func, IHC, IHC-Fr, IP, WB

Usage: Flow Cytometry: Recommended dilution - 4 μg/mL. Immunohistochemistry (frozen sections): It is suitable for discrimination of lymphomas from other tumors. Functional Application: The antibody MEM-53 induces activation of monocytes and B lymphocytes.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CD53

Target: CD53

Alternative Name: CD53 (CD53 Products)

Background: Name/Gene ID: CD53
Family: Tetraspan

Synonyms: CD53, CD53 antigen, CD53 Molecule, CD53 tetraspan antigen, Cell surface antigen, Leukocyte surface antigen CD53, Transmembrane glycoprotein, Tetraspanin-25, Tspan-25, CD53 glycoprotein, Cell surface glycoprotein CD53, MOX44, TSPAN25

Gene ID: 963

UniProt: P19397