CD300a antibody

Catalog No. ABIN207134

The Mouse Monoclonal anti-CD300a antibody has been validated for WB, FACS and Inhibition. It is suitable to detect CD300a in samples from Human.

Quick Overview for CD300a antibody (ABIN207134)

Target: CD300a (CD300A)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD300a antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Blocking Antibody (Inhibition)

Clone: MEM-260

Product Details anti-CD300a Antibody

Specificity: Reacts with CD300a, a 60 kD leukocyte transmembrane glycoprotein (immunoglobulin superfamily), expressed on human granulocytes, monocytes, neutrophils, NK cells, 25 % of circulating T cells and 15 % of circulating B cells. Expression is also on T and B subsets, B cells, dendritic cells and mast cells.

Purification: Protein A purified

Immunogen: HPB human acute lymphoid leukemia cell line.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, Inhb, WB

Usage: Flow Cytometry: Recommended dilution - 2 μg/mL. Western Blotting: Positive material: HPB cell line. Negative material: JURKAT human cell line, RAJI human cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mL cold Lysis buffer (1 % laurylma

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CD300a

Target: CD300a (CD300A)

Alternative Name: CD300A

Background: Name/Gene ID: CD300A

Synonyms: CD300A, CD300a antigen, CD300a molecule, CMRF35-like molecule 8, CMRF35-H, CMRF35-H9, CLM-8, CMRF35H9, IGSF12, Inhibitory receptor protein 60, IRC2, IRC1, NK inhibitory receptor, CMRF-35-H9, CMRF-35H, CMRF35H, IRC1a, IRp60, Leukocyte membrane antigen

Gene ID: 11314

UniProt: Q9UGN4

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process