CD8 antibody (Biotin)

Catalog No. ABIN207164

The Mouse Monoclonal anti-CD8 antibody has been validated for FACS and IF. It is suitable to detect CD8 in samples from Human.

Quick Overview for CD8 antibody (Biotin) (ABIN207164)

Target: CD8

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: MEM-31

Product Details anti-CD8 Antibody (Biotin)

Specificity: Recognizes a conformationally-dependent epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8alpha homodimer or CD8alpha/beta heterodimer (each monomer approx. 32-34 kD). The antibody does not react with formaldehyde-fixed cells, negative in Western Blot application.

Purification: Protein A purified

Immunogen: Crude thymus membrane fraction.

Type of Immunogen: Membrane fraction

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:2500), IF (1:2500)

Usage: Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working dilution is 1:2500. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by th The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CD8

Target: CD8

Alternative Name: CD8

UniProt: P01732