CD74 antibody (C-Term) (FITC)

Catalog No. ABIN207706

This Mouse Monoclonal antibody specifically detects CD74 in FACS. It exhibits reactivity toward Human.

Quick Overview for CD74 antibody (C-Term) (FITC) (ABIN207706)

Target: CD74 (HLA-DR-gamma (CD74))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD74 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: BU45

Product Details anti-CD74 Antibody (FITC)

Binding Specificity: C-Term

Specificity: Recognizes human CD74, a type II transmembrane protein, which is also known as the MHC class II associated invariant chain. The Recognizes 33, 35 and 41 kD isoforms of CD74. The CD74 Molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes. CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, dendritic cells and by various types of carcinoma. Expression of CD74 is primarily intracellular with moderate expression at the cell surface of B cells and monocytes. The Bu45 antibody binds to an epitope within the extracytoplasmic domain of the CD74 Molecule, which is distal from the C-terminus. *Membrane permeabilization may enhance CD74 staining on monocytes but is not required when staining lymphocytes. XXX recommend the use of Leucoperm (product code BUF09) for this purpose.

Purification: Protein G purified

Immunogen: B lymphoblastoid cell line HFB1.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:1 - 1:5)

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for CD74

Target: CD74 (HLA-DR-gamma (CD74))

Alternative Name: CD74 / CLIP

Background: Name/Gene ID: CD74

Synonyms: CD74, CD74 antigen, DHLAG, HLADG, HLA-DR-gamma, Ia-associated invariant chain, p33, MHC HLA-DR gamma chain, Ia-GAMMA

Gene ID: 972

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints