Claudin 1 antibody (C-Term)

Catalog No. ABIN208270

This Mouse Monoclonal antibody specifically detects Claudin 1 in WB, IHC, ELISA, IF, IP and IHC (fro). It exhibits reactivity toward Human, Rat, Mouse and Dog.

Quick Overview for Claudin 1 antibody (C-Term) (ABIN208270)

Target: Claudin 1 (CLDN1)

Reactivity: Human, Rat, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Claudin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2H10D10

Product Details anti-Claudin 1 Antibody

Binding Specificity: C-Term

Specificity: Specific for the C-terminal region of the human claudin-1 protein. On Western Blots, it identifies the target band at ~22kD. Reactivity has been confirmed with human Caco-2 and canine MDCK cell lysates, mouse kidney and intestinal lysates, frozen mouse intestine (jejunum) tissue, and rat kidney homogenates.

Purification: Protein A purified

Immunogen: Synthetic peptide derived from the C-terminal region of the human and mouse claudin-1 proteins.

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (1 - 5 μg/mL), IHC, IHC-Fr, IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. ELISA: 0.1-1 μg/mL. Western Blot: 1-3 μg/mL. Immunofluorescence: 1-5 μg/mL. Immunoprecipitation: 10 μg/test. Immunohistochemistry (Frozen): 5-10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Claudin 1

Target: Claudin 1 (CLDN1)

Alternative Name: CLDN1 / Claudin 1

Background: Name/Gene ID: CLDN1
Family: Claudin

Synonyms: CLDN1, Claudin-1, Claudin 1, ILVASC, SEMP1, CLD1

Gene ID: 9076

UniProt: O95832

Pathways: Cell-Cell Junction Organization, Hepatitis C