Claudin 1 antibody (C-Term)

Catalog No. ABIN208271

The Rabbit Polyclonal anti-Claudin 1 antibody has been validated for WB, IHC, ELISA, IF and IHC (p). It is suitable to detect Claudin 1 in samples from Human, Rat and Dog.

Quick Overview for Claudin 1 antibody (C-Term) (ABIN208271)

Target: Claudin 1 (CLDN1)

Reactivity: Human, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claudin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Claudin 1 Antibody

Binding Specificity: C-Term

Specificity: Recognizes the ~22kD Claudin-1 protein, and does not cross-react with Claudin-3. Reactivity has been confirmed with human Caco-2 and canine MDCK cell lysates and rat kidney homogenates by Western Blot and immunofluorescence, and with human skin tissue by immunohistochemistry.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the C-terminus of the human Claudin-1 protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (5 - 10 μg/mL), IHC, IHC-P (5 - 10 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. ELISA (Immunogen): 0.1-1 μg/mL. Western Blot (Canine, Human): 1-2 μg/mL. Immunofluorescence (Rat): 5-10 μg/mL. Immunohistochemistry (Human): 5-10 μg/mL, For best results in IHC with formalin-fixed, paraffin-embedded tissues, heat induced epitope retrieval (HIER) with EDTA,  pH 8.0, is required prior to staining.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Claudin 1

Target: Claudin 1 (CLDN1)

Alternative Name: CLDN1 / Claudin 1

Background: Name/Gene ID: CLDN1
Family: Claudin

Synonyms: CLDN1, Claudin-1, Claudin 1, ILVASC, SEMP1, CLD1

Gene ID: 9076

UniProt: O95832

Pathways: Cell-Cell Junction Organization, Hepatitis C