Cullin 1 antibody (C-Term)

Catalog No. ABIN208312

This Rabbit Polyclonal antibody specifically detects Cullin 1 in WB, ELISA and IP. It exhibits reactivity toward Human.

Quick Overview for Cullin 1 antibody (C-Term) (ABIN208312)

Target: Cullin 1 (CUL1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-Cullin 1 Antibody

Binding Specificity: C-Term

Specificity: Specific for the 86kD CUL-1 protein and does not cross-react with the related CUL2 protein. Antibody reactivity has been confirmed by Western blotting and immunoprecipitation using both recombinant CUL1 protein and cell lysates. Reacts with human CUL1. Reactivity with other species is possible based on sequence homology but has not been tested.

Purification: Immunoaffinity purified

Immunogen: An 11 amino acid synthetic peptide derived from the extreme C-terminus of the human CUL1 protein. This peptide sequence is identical to Drosophila CUL1 (LIN-19) in 9/11 positions and in 7/11 residues with the CUL1 C. elegans and Dictyostelium discoideum homologs.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation. ELISA: 0.1-1.0 μg/mL. Western Blot: 1-3 μg/mL. Immunoprecipitation: 5-10 μg.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Cullin 1

Target: Cullin 1 (CUL1)

Alternative Name: Cullin 1 / CUL1

Background: Name/Gene ID: CUL1

Synonyms: CUL1, Cullin 1, Cullin-1, CUL-1

Gene ID: 8454

UniProt: Q13616

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases, Regulation of Cell Size