FZD8 antibody (Extracellular Domain)

Catalog No. ABIN6757419

This Rat Monoclonal antibody specifically detects FZD8 in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for FZD8 antibody (Extracellular Domain) (ABIN6757419)

Target: FZD8 (Frizzled Family Receptor 8 (FZD8))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This FZD8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FZD8 Antibody

Binding Specificity: Extracellular Domain

Specificity: Recognizes mouse Frizzled-8. In Direct ELISAs, shows approximately 15 % cross-reactivity with rmFrizzled-6 and no cross-reactivity with rmFrizzled-1, -2, -3, -4, or -7.

Purification: Protein G purified

Immunogen: NS0-derived rmFrizzled-8 extracellular domain (CRD).

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Direct ELISA. Western Blot: Use at 1-2 μg/mL. Using a colorimetric detection system, the detection limit for rmFrizzled-8 is ~25 ng/lane under reducing conditions. Direct ELISA: Use at 0.5-1.0 μg/mL. The detection limit is ~5 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 5 % trehalose, 40 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for FZD8

Target: FZD8 (Frizzled Family Receptor 8 (FZD8))

Alternative Name: FZD8 / Frizzled 8

Background: Name/Gene ID: FZD8
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD8, Frizzled family receptor 8, Frizzled homolog 8, FZ-8, Fz8, Frizzled 8, Frizzled-8, HFZ8

Gene ID: 8325

UniProt: Q9H461

Pathways: WNT Signaling