MMP3 antibody

Catalog No. ABIN209004

This anti-MMP3 antibody is a Mouse Monoclonal antibody detecting MMP3 in WB, IHC, IP, IF, IHC (p) and IHC (fro). Suitable for Human.

Quick Overview for MMP3 antibody (ABIN209004)

Target: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MMP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MMP3 Antibody

Specificity: Recognizes one doublet of 54kD/59kD and another doublet of 44kD/49kD, which are identified as unglycosylated and glycosylated species of pro (latent) and active forms of matrix metalloproteinase-3 (MMP-3, also known as Stromelysin-1 of Transin). No cross-reactivity with the pro and active forms of other MMPs. Recognizes human.

Purification: Protein A purified

Immunogen: APMA (4-Aminophenylmercuric acetate) activated Human stromelysin-1.

Isotype: IgG2b

Application Details

Application Notes: Approved: IF, IHC, IHC-Fr, IHC-P, IP (2 μg/mL), WB (0.5 - 1 μg/mL)

Usage: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Use Protein A): 2 μg/mL Protein lysate. Western Blot: 0.5-1.0 μg/mL for 2hrs at RT. Immunohistochemistry (frozen & formalin-fixed):10-20g/mL for 60 minute at RT. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues. Positive control: Human endometrium cells, placenta, bladder, breast, ovarian carcinomas.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for MMP3

Target: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Alternative Name: MMP3 / MMP-3

Background: Name/Gene ID: MMP3
Subfamily: Metallopeptidase M10A
Family: Protease

Synonyms: MMP3, CHDS6, MMP-3, Prostromelysin-1, Proteoglycanase, SL-1, STR1, Matrix metalloproteinase-3, Transin, Transin-1, SLN-1, STMY, Progelatinase, STMY1, Stromelysin-1

Gene ID: 4314

UniProt: P08254