OCT4 antibody (AA 282-297)

Catalog No. ABIN209117

The Rabbit Polyclonal anti-OCT4 antibody is suitable to detect OCT4 in samples from Human and Rodents. It has been validated for WB, IHC and ICC.

Quick Overview for OCT4 antibody (AA 282-297) (ABIN209117)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human, Rodents

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-OCT4 Antibody

Binding Specificity: AA 282-297

Specificity: Recognizes Oct4 (POU5F1). MW: 38,.6kD. Species cross-reactivity: Human and rodent.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide (aa 282-297) of Oct4.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:200 - 1:1000), IHC (1:200 - 1:1000), WB (1:200 - 1:1000)

Usage: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:200-1:1000 using ECL. Immunohistochemistry: 1:200-1:1000. Immunocytochemistry: 1:200-1:1000.Immunohistochemistry protocol:1. Dissect tissues and freeze on dry ice.2. Cut on a cryostat 10um slices.3. Dry slides at RT for 30-90 min4. Fix with cold Acetone: Methanol (50:50) 2 min. Either process further or store at -20 °C. Alternative is fixation with 4 % PFA and treatment with 0.05 % trypsin solution (incubation 10 min. at 37 °C, followed by 3 washes with PBS)5. Let air dry.6. PBS 5 min7. Incubate in 50  mM ammonium chloride 30 min.8. PBS 5 min9. Blocking serum 30-45 min10. Primary antibody (LS-C9379) 90 min11. Wash 3X for 5 min12. Secondary fluorescent conjugated antibody 30 min13. Wash 3X for 5 min.14. Mount.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4. No preservative added.

Preservative: Without preservative

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: POU5F1 / OCT4

Background: Name/Gene ID: POU5F1

Synonyms: POU5F1, Oct-4, OCT3, OCT4, OTF4, POU class 5 homeobox 1, Octamer-binding protein 3, Octamer-binding protein 4, OTF-3, Oct-3, OTF3

Gene ID: 5460

UniProt: Q01860

Pathways: Stem Cell Maintenance