CD8 alpha antibody

Catalog No. ABIN210181

This Mouse Monoclonal antibody specifically detects CD8 alpha in FACS, IHC, WB, IP, IHC (fro) and IHC (p). It exhibits reactivity toward Rat.

Quick Overview for CD8 alpha antibody (ABIN210181)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD8 alpha Antibody

Specificity: Recognizes the rat CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells. Clone MRC OX-8 is suitable for use in in vitro blocking studies. XXX recommend the use of MCA48XZ for this purpose. * This clone has been described reacting with paraffin-embedded material following PLP Fixation (periodate-lysine paraformaldehyde) (3) This product is routinely tested in flow cytometry on rat splenocytes.

Purification: Protein G purified

Immunogen: Rat thymocyte membrane glycoproteins.

Type of Immunogen: Membrane extract

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:100), IHC, IHC-Fr, IHC-P, IP, WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin embedded sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8A / CD8 alpha

Background: Name/Gene ID: CD8A

Synonyms: CD8A, CD8a molecule, CD8a antigen, OKT8 T-cell antigen, Leu2 T-lymphocyte antigen, T8 T-cell antigen, T cell co-receptor, p32, CD8, Leu2, T-cell antigen Leu2

Gene ID: 925

Pathways: TCR Signaling