EHMT2 antibody (AA 71-88)

Catalog No. ABIN210887

The Rabbit Polyclonal anti-EHMT2 antibody has been validated for WB. It is suitable to detect EHMT2 in samples from Human and Monkey.

Quick Overview for EHMT2 antibody (AA 71-88) (ABIN210887)

Target: EHMT2 (Ankyrin Repeat-Containing Protein (EHMT2))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EHMT2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EHMT2 Antibody

Binding Specificity: AA 71-88

Specificity: Recognizes human G9a protein, Mr ~140kD. Species cross-reactivity: Human. Predicted to cross-react with mouse based on sequence homology.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%) Mouse, Rat, Elephant, Panda, Bovine, Horse, Rabbit, Pig (94%).

Purification: Protein A purified

Immunogen: KLH-conjugated, synthetic peptide (C-DERVDSDSKSEVEALTEQ) corresponding to amino acids 71-88 of human G9a, with an N-terminal cysteine added for conjugation purposes. The immunizing sequence has 17/18 identical amino acids in mouse. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%), Mouse, Rat, Elephant, Panda, Bovine, Horse, Rabbit, Pig (94%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 2 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot: 0.2-2 μg/mL detected G9a in HeLa nuclear extract cell lysate. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-G9a (0.5 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for EHMT2

Target: EHMT2 (Ankyrin Repeat-Containing Protein (EHMT2))

Alternative Name: EHMT2 / G9A

Background: Name/Gene ID: EHMT2

Synonyms: EHMT2, BAT8, C6orf30, Em:AF134726.3, KMT1C, G9A, GAT8, H3-K9-HMTase 3, Protein G9a, G9A histone methyltransferase, HLA-B associated transcript 8, HLA-B-associated transcript 8, Lysine N-methyltransferase 1C, NG36

Gene ID: 10919

UniProt: Q96KQ7