TYR antibody (AA 5-456)

Catalog No. ABIN210982

This Mouse Monoclonal antibody specifically detects TYR in WB, IHC, IHC (p) and IP. It exhibits reactivity toward Human.

Quick Overview for TYR antibody (AA 5-456) (ABIN210982)

Target: TYR (Tyrosinase (TYR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TYR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-TYR Antibody

Binding Specificity: AA 5-456

Specificity: Recognizes Tyrosinase, Mr 70-80kD. An unknown band at ~55kD may also be detected in SKMEL- 19 cell lysates. Species cross-reactivity: Human. Predicted to cross-react with many species based on extensive sequence homology.

Purification: Protein G purified

Immunogen: Purified recombinant Tyrosinase corresponding to residues 5-456 of human Tyrosinase.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: IHC, IHC-P, IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Not suitable for use in Immunoprecipitation. Western Blot: 0.5-2 μg/mL detects Tyrosinase in RIPA lysates from SK-MEL-19 cells. Immunohistochemistry: Detects melanocytic lesions in paraffin embedded tissues.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TYR

Target: TYR (Tyrosinase (TYR))

Alternative Name: Tyrosinase

Background: Name/Gene ID: TYR

Synonyms: TYR, CMM8, LB24-AB, SHEP3, SK29-AB, Monophenol monooxygenase, Tumor rejection antigen AB, OCA1A, Tyrosinase, OCAIA

Gene ID: 7299

UniProt: P14679