Calreticulin antibody (C-Term)

Catalog No. ABIN211075

This Rabbit Polyclonal antibody specifically detects Calreticulin in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Pig, Cow and Xenopus laevis.

Quick Overview for Calreticulin antibody (C-Term) (ABIN211075)

Target: Calreticulin (CALR)

Reactivity: Human, Mouse, Rat, Pig, Cow, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calreticulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Calreticulin Antibody

Binding Specificity: AA 412-417, C-Term

Specificity: Specific for calreticulin. Does not cross-react with other endoplasmic reticulum proteins. Species cross-reactivity: Human, mouse, rat, Xenopus and weakly with bovine and porcine. No reaction with chicken.

Purification: Protein A purified

Immunogen: Synthetic peptide (QAKDEL) corresponding to the C-terminus (amino acids 412-417) of human calreticulin, KLH conjugated.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, WB (0.5 - 2 μg/mL)

Usage: Suitable for Western Blot and Immunohistochemistry. Western Blot: 0.5-2 μg/mL detects calreticulin in RIPA lysates from murine 3T3/NIH fibroblasts and human foreskin fibroblast (HFF). 20 μg 3T3/NIH and 10 μg HFF cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-human Calreticulin (0.5 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunohistochemistry: Detects calreticulin in tissue sections and cells. Included Positive Antigen Control: 3T3 lysate. Use 20 μg per lane for minigels.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Frozen solution in 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Calreticulin

Target: Calreticulin (CALR)

Alternative Name: CALR / Calreticulin

Background: Name/Gene ID: CALR

Synonyms: CALR, Calregulin, CC1qR, CRP55, CRT, CRTC, ERp60, Grp60, RO, SSA, Calreticulin, HACBP

Gene ID: 811

UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response