CELF1 antibody

Catalog No. ABIN211134

This anti-CELF1 antibody is a Mouse Monoclonal antibody detecting CELF1 in WB, IP and ICC. Suitable for Human, Mouse, Rat, Cow, Rabbit and Pig.

Quick Overview for CELF1 antibody (ABIN211134)

Target: CELF1 (CUGBP, Elav-Like Family Member 1 (CELF1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CELF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-CELF1 Antibody

Specificity: Recognizes human CUG-BP1, Mr 50kD. Species cross-reactivity: Reported to cross-react with mouse, rat, bovine, porcine and rabbit.

Purification: Protein G purified

Immunogen: Full-length GST fusion protein corresponding to human CUG-BP1, also known as heterogeneous nuclear ribonucleoprotein (hnRNP) hNab50.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IP, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Supershift Assay. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: Immunoprecipitates CUG-BP1 from HeLa cell lysates. Immunocytochemistry: Nuclear staining in HeLa and myoblasts from normal patients. Supershift: Supershifts CUG-BP1: DNA complexes in EMSA.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.07 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CELF1

Target: CELF1 (CUGBP, Elav-Like Family Member 1 (CELF1))

Alternative Name: CELF1 / CUGBP1

Background: Name/Gene ID: CELF1

Synonyms: CELF1, Bruno-like protein 2, BRUNOL2, Bruno-like 2, CELF-1, CUG RNA-binding protein, CUGBP1, CUG-BP, EDEN-BP, HNab50, NAB50, NAPOR, RNA-binding protein BRUNOL-2, CUG-BP1, CUGBP, Deadenylation factor CUG-BP, EDEN-BP homolog

Gene ID: 10658

UniProt: Q92879

Pathways: Ribonucleoprotein Complex Subunit Organization