CELF1 antibody

Catalog No. ABIN211134

The Mouse Monoclonal anti-CELF1 antibody (ABIN211134) specifically detects CELF1 in WB, IP and ICC. The antibody is reactive with Human, Mouse, Rat, Cow, Rabbit and Pig samples.

Quick Overview for CELF1 antibody (ABIN211134)

Target: CELF1 (CUGBP, Elav-Like Family Member 1 (CELF1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CELF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-CELF1 Antibody

Specificity: Recognizes human CUG-BP1, Mr 50kD. Species cross-reactivity: Reported to cross-react with mouse, rat, bovine, porcine and rabbit.

Purification: Protein G purified

Immunogen: Full-length GST fusion protein corresponding to human CUG-BP1, also known as heterogeneous nuclear ribonucleoprotein (hnRNP) hNab50.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IP, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Supershift Assay. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: Immunoprecipitates CUG-BP1 from HeLa cell lysates. Immunocytochemistry: Nuclear staining in HeLa and myoblasts from normal patients. Supershift: Supershifts CUG-BP1: DNA complexes in EMSA.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.07 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CELF1

Target: CELF1 (CUGBP, Elav-Like Family Member 1 (CELF1))

Alternative Name: CELF1 / CUGBP1

Background: Name/Gene ID: CELF1

Synonyms: CELF1, Bruno-like protein 2, BRUNOL2, Bruno-like 2, CELF-1, CUG RNA-binding protein, CUGBP1, CUG-BP, EDEN-BP, HNab50, NAB50, NAPOR, RNA-binding protein BRUNOL-2, CUG-BP1, CUGBP, Deadenylation factor CUG-BP, EDEN-BP homolog

Gene ID: 10658

UniProt: Q92879

Pathways: Ribonucleoprotein Complex Subunit Organization