FAS antibody (Cytoplasmic Domain)

Catalog No. ABIN211156

This Rat Monoclonal antibody specifically detects FAS in WB and IP. It exhibits reactivity toward Mouse and Cow.

Quick Overview for FAS antibody (Cytoplasmic Domain) (ABIN211156)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Mouse, Cow

Host: Rat

Clonality: Monoclonal

Conjugate: This FAS antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-FAS Antibody

Binding Specificity: Cytoplasmic Domain

Specificity: Recognizes mouse Fas at about 45kD. Species cross-reactivity: bovine.

Purification: Protein G purified

Immunogen: Recombinant protein containing the cytoplasmic domain of mouse Fas.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-2 μg/mL detects Fas (~45 kD) in RIPA lysates of mouse 3T3/A31 fibroblast cells and bovine brain. Immunoprecipitation: 4 μg immunoprecipitates Fas from 3T3 cells. RIP, a death domain containing protein, was co-precipitated with Fas.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine, 0.15 M sodium chloride,  pH 7.4, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: CD95 / FAS

Background: Name/Gene ID: FAS
Family: TNF Receptor

Synonyms: FAS, ALPS1A, APO-1, APT1, Apo-1 antigen, APO-1 cell surface antigen, Apo11 antigen, Apoptosis antigen 1, CD95 antigen, Delta Fas/APO-1/CD95, Fas AMA, FAS1, Fasl receptors, FASLG receptor, FASTM, FAS 827dupA, Fasl receptor, TNFRSF6, CD95

Gene ID: 355

UniProt: P25445

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity