CYBB antibody (AA 548-560)

Catalog No. ABIN211171

This Rabbit Polyclonal antibody specifically detects CYBB in WB. It exhibits reactivity toward Human, Mouse, Rat, Bat and Monkey.

Quick Overview for CYBB antibody (AA 548-560) (ABIN211171)

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Reactivity: Human, Mouse, Rat, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYBB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CYBB Antibody

Binding Specificity: AA 548-560

Specificity: Recognizes human gp91 phox, Mr 75kD. Depending on the type of lysate, the amount loaded, the gel and the blot conditions, gp91 phox may run as a sharp band, Mr ~75kD, or appear as a smear between 75-91kD. Species sequence Homology: mouse-100 % , bovine-11/13, porcine-10/13.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bat (100%) Elephant, Panda, Dog (92%) Dolphin, Bovine, Horse, Turkey, Chicken, Platypus (85%).

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to aa548-560 of human gp91-phox (KQSISNSESGPRG) (KLH-conjugated). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bat (100%), Elephant, Panda, Dog (92%), Dolphin, Bovine, Horse, Turkey, Chicken, Platypus (85%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: WB (1 - 4 μg/mL)

Usage: Suitable for use in Western Blot. (4 μg/mL). Proteins were visualized using a secondary antibody IgG (HRP) Pab Gt x Rb* and a chemiluminescence detection system. Positive control: Lung tissue, human neutrophil membrane, HL60 membrane and RIPA cell lysate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.07 M Tris-glycine,  pH 7.4, 0.105 M sodium chloride, 0.035 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CYBB

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Alternative Name: NOX2 / gp91phox

Background: Name/Gene ID: CYBB

Synonyms: CYBB, Chronic granulomatous disease, CGD91-phox, Cytochrome b558 subunit beta, Cytochrome b(558) subunit beta, gp91-1, gp91-PHOX, p22 phagocyte B-cytochrome, AMCBX2, CGD, Cytochrome b-245 heavy chain, gp91PHOX, NADPH oxidase 2, NOX2, p91-PHOX

Gene ID: 1536

UniProt: P04839