SMC1A antibody (pSer957)

Catalog No. ABIN211403

The Mouse Monoclonal anti-SMC1A antibody has been validated for WB. It is suitable to detect SMC1A in samples from Human, Cow, Dog, Rabbit and Monkey.

Quick Overview for SMC1A antibody (pSer957) (ABIN211403)

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Reactivity: Human, Cow, Dog, Rabbit, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMC1A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SMC1A Antibody

Binding Specificity: AA 951-962, pSer957

Specificity: Recognizes phospho-SMC1 (Ser957), Mr 150kD. Species cross-reactivity: Human and mouse. Predicted to cross-react with Xenopus and bovine based on sequence homology.

Purification: Protein G purified

Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 951-962 SQEEGS[pS]QGEDS) of human SMC1 (Structural Maintenance of Chromosome 1). The immunizing sequence is identical in bovine and has 11/12 identical amino acids in mouse and xenopus.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot: 1-2 μg/mL detects phospho-SMC1 in RIPA lysates from irradiated GMO536 cells and hydroxyurea treated HEK293 cells. Cell lysates from untreated and irradiated GMO536 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-SMC1 (Ser957) (2 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for SMC1A

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Alternative Name: SMC1A / SMC1

Background: Name/Gene ID: SMC1A

Synonyms: SMC1A, CDLS2, DXS423E, KIAA0178, SB1.8, SMC protein 1A, SMC1, SMC1L1, SMC-1-alpha, SMC-1A, SMCB

Gene ID: 8243

UniProt: Q14683

Pathways: Stem Cell Maintenance