Fc gamma RII (CD32) antibody

Catalog No. ABIN7470726

This anti- antibody is a Mouse Monoclonal antibody detecting in FACS, IHC, IP and IHC (fro). Suitable for Human, Dog, Rhesus Monkey and Pig.

Quick Overview for Fc gamma RII (CD32) antibody (ABIN7470726)

Target: Fc gamma RII (CD32)

Reactivity: Human, Dog, Rhesus Monkey, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: AT10

Product Details

Specificity: Recognizes the human CD32 antigen, a ~40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Clone AT10 blocks the binding of IgG to Fc gamma RII.

Purification: Protein A purified

Immunogen: K562 cell line.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (20 μg/mL), IHC, IHC-Fr (1:500 - 1:1000), IP (20 μg/mL)

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells or cells or 100 μL whole blood. Method sheets are available on request.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: Fc gamma RII (CD32)

Alternative Name: FCGR2 / CD32