ECE1 antibody (AA 214-448)

Catalog No. ABIN2117822

The Rabbit Polyclonal anti-ECE1 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect ECE1 in samples from Rat.

Quick Overview for ECE1 antibody (AA 214-448) (ABIN2117822)

Target: ECE1 (Endothelin-Converting Enzyme 1 (ECE1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ECE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-ECE1 Antibody

Binding Specificity: AA 214-448

Purpose: Polyclonal Antibody to Endothelin Converting Enzyme 1 (ECE1)

Specificity: The antibody is a rabbit polyclonal antibody raised against ECE1. It has been selected for its ability to recognize ECE1 in immunohistochemical staining and western blotting.

Characteristics: The antibody is a rabbit polyclonal antibody raised against ECE1. It has been selected for its ability to recognize ECE1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: ECE1 (His214-Ala448)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ECE1

Target: ECE1 (Endothelin-Converting Enzyme 1 (ECE1))

Alternative Name: ECE1

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling