Actin antibody (pan)

Catalog No. ABIN2132953

The Mouse Monoclonal anti-Actin antibody has been validated for WB, IHC (p), IP and IHC (fro). It is suitable to detect Actin in samples from Eukaryotes. There is 1 publication available.

Quick Overview for Actin antibody (pan) (ABIN2132953)

Target: Actin

Reactivity: Eukaryotes

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: ACTN05

Product Details anti-Actin Antibody

Binding Specificity: pan

Cross-Reactivity: Chicken, Cow (Bovine), Dog (Canine), Dictyostelium, Human, Mouse (Murine), Slime mold (Physarum polycephalum), Pig (Porcine), Rabbit, Rat (Rattus)

Purification: Purified by Protein G chromatography

Immunogen: Actin-pan antibody was raised in mouse using chicken gizzard actin as the immunogen.

Isotype: IgG1 kappa

Application Details

Application Notes: IHC-F: 1:50, IHC-P: 1:50, IP: 2 μg/mg protein lysate, WB: 0.5 μg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % Sodium azide.

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4 °C.

References for anti-Actin antibody (ABIN2132953)

Shimabukuro Okuda, Tallada Iborra, Ramos Pinto, Fabres Machado, Corrêa-Giannella, Pickford, Woods, Brimble, Rye, Passarelli: "Advanced Glycated apoA-IV Loses Its Ability to Prevent the LPS-Induced Reduction in Cholesterol Efflux-Related Gene Expression in Macrophages." in: Mediators of inflammation, Vol. 2020, pp. 6515401, (2020) (PubMed).

Target Details for Actin

Target: Actin