GPR175 antibody (Cytoplasmic Domain)

Catalog No. ABIN213352

This Rabbit Polyclonal antibody specifically detects GPR175 in IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Horse, Cow, Pig, Bat and Hamster.

Quick Overview for GPR175 antibody (Cytoplasmic Domain) (ABIN213352)

Target: GPR175 (TPRA1) (Transmembrane Protein, Adipocyte Asscociated 1 (TPRA1))

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Pig, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR175 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GPR175 Antibody

Binding Specificity: Cytoplasmic Domain

Brand: IHC-plus™

Specificity: Human TPRA1 / GPR175. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Dog, Bovine, Bat, Hamster, Elephant, Panda, Horse, Pig, Platypus (100%) Monkey, Rabbit, Opossum, Turkey, Lizard, Zebrafish (94%) Chicken, Seabass, Salmon, Stickleback (88%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 17 amino acid peptide from 2nd cytoplasmic domain of human TPRA1 / GPR175. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Dog, Bovine, Bat, Hamster, Elephant, Panda, Horse, Pig, Platypus (100%), Monkey, Rabbit, Opossum, Turkey, Lizard, Zebrafish (94%), Chicken, Seabass, Salmon, Stickleback (88%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 - 20 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GPR175

Target: GPR175 (TPRA1) (Transmembrane Protein, Adipocyte Asscociated 1 (TPRA1))

Alternative Name: TPRA1 / GPR175

Background: Name/Gene ID: TPRA1
Subfamily: Orphan-U
Family: GPCR

Synonyms: TPRA1, G protein-coupled receptor 175, Transmembrane protein 227, TMEM227, TPRA40, GPR175

Gene ID: 131601