GPR81 antibody (N-Term)

Catalog No. ABIN213368

The Rabbit Polyclonal anti-GPR81 antibody has been validated for ELISA, IHC and IHC (p). It is suitable to detect GPR81 in samples from Human and Monkey.

Quick Overview for GPR81 antibody (N-Term) (ABIN213368)

Target: GPR81 (G Protein-Coupled Receptor 81 (GPR81))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR81 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GPR81 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human GPR81. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Monkey (100%) Gorilla (95%) Marmoset (89%) Dog, Hamster, Elephant (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 19 amino acid peptide from N-terminal extracellular domain of human GPR81. Percent identity with other species by BLAST analysis: Human, Monkey (100%), Gorilla (95%), Marmoset (89%), Dog, Hamster, Elephant (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (20 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GPR81

Target: GPR81 (G Protein-Coupled Receptor 81 (GPR81))

Alternative Name: FKSG80 / GPR81

Background: Name/Gene ID: HCAR1
Subfamily: Chemokine
Family: GPCR

Synonyms: HCAR1, FKSG80, G protein-coupled receptor 81, G-protein coupled receptor 104, G protein-coupled receptor 104, G-protein coupled receptor 81, GPR81, GPR104, LACR1, HCA1, TA-GPCR, Lactate receptor 1

Gene ID: 27198