GPR81 antibody (C-Term, Intracellular)

Catalog No. ABIN7886507

This Rabbit Polyclonal antibody specifically detects GPR81 in WB and IHC. It exhibits reactivity toward Mouse.

Quick Overview for GPR81 antibody (C-Term, Intracellular) (ABIN7886507)

Target: GPR81 (G Protein-Coupled Receptor 81 (GPR81))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR81 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GPR81 Antibody

Binding Specificity: AA 319-332, C-Term, Intracellular

Purpose: A Rabbit Polyclonal Antibody to GPR81

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): The antibody will not recognize human GPR81 (HCAR1).

Predicted Reactivity: Rat - 9,14 amino acid residues identical

Characteristics: Anti-GPR81/HCAR1 Antibody (ABIN7043173, ABIN7044514 and ABIN7044515) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize HCAR1 from rat and mouse samples. The antibody will not recognize human GPR81.

Purification: Affinity purified on immobilized antigen.

Immunogen: CSKSSIDGANRSQR, corresponding to amino acid residues 319-332 of mouse GPR81 (HCAR1)

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235445)

Blocking Peptide: (ABIN7235445)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GPR81

Target: GPR81 (G Protein-Coupled Receptor 81 (GPR81))

Alternative Name: HCAR1

Background:

Synonyms: Hydroxycarboxylic acid receptor 1, HCA1 Receptor, G-protein coupled receptor 81, GPR81, G Protein-Coupled Receptor 104, LACR1

Description: GPR81 is an orphan G-protein-coupled receptor that belongs to a family of hydroxy-carboxylic acid (HCA) receptors. Its endogenous ligand is L-lactate, a metabolite produced when glycolysis exceeds the capacity to oxidize pyruvate1,2.In addition to being a by-product of metabolism, lactate plays a role in insulin signaling, particularly in insulin mediated anti-lipolytic effects. Likewise, GPR81 is mostly expressed in adipose tissues and its activation results in inhibition of lipolysis and reduced hydrolysis of triglycerides to glycerol and fatty acids, suggesting that GPR81 may be an attractive drug discovery target for metabolic disorders4.Beyond the control of adipocyte lipolysis, GPR81 is also involved in neuronal signaling, regulation of blood flow to the retina. The receptor is overexpressed in several cancer types where it appears to play a role in the adaptation to the ischemic tumor microenvironment5.GPR81, like all other G protein coupled receptors, consists of seven transmembrane domains, in addition it possesses a highly conserved Cys-Glu-Ser-Phe motif in the second extracellular loop, essential for its function and six Cys residues at the extracellular domains that potentially form three disulfide bonds6.

Gene ID: 243270

NCBI Accession: NM_032554

UniProt: Q8C131