GPR153 antibody (C-Term)

Catalog No. ABIN213427

This Rabbit Polyclonal antibody specifically detects GPR153 in ELISA, IHC and IHC (p). It exhibits reactivity toward Human and Monkey.

Quick Overview for GPR153 antibody (C-Term) (ABIN213427)

Target: GPR153 (G Protein-Coupled Receptor 153 (GPR153))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR153 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GPR153 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human GPR153. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except PCDHGA2 (56 % ), PCDHGA4 (50 %).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Monkey (100%) Rat (94%) Marmoset, Mouse, Bovine, Hamster, Elephant (88%) Bat, Dog, Pig (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from C-terminal cytoplasmic domain of human GPR153. Percent identity with other species by BLAST analysis: Human, Monkey (100%), Rat (94%), Marmoset, Mouse, Bovine, Hamster, Elephant (88%), Bat, Dog, Pig (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (34 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 17 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GPR153

Target: GPR153 (G Protein-Coupled Receptor 153 (GPR153))

Alternative Name: PGR1 / GPR153

Background: Name/Gene ID: GPR153
Subfamily: Orphan-U
Family: GPCR

Synonyms: GPR153, PGR1, G protein-coupled receptor 153

Gene ID: 387509