GPR174 antibody (Cytoplasmic Domain)

Catalog No. ABIN213461

The Rabbit Polyclonal anti-GPR174 antibody has been validated for ELISA, IHC and IHC (p). It is suitable to detect GPR174 in samples from Human and Monkey.

Quick Overview for GPR174 antibody (Cytoplasmic Domain) (ABIN213461)

Target: GPR174 (G Protein-Coupled Receptor 174 (GPR174))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR174 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GPR174 Antibody

Binding Specificity: Cytoplasmic Domain

Brand: IHC-plus™

Specificity: Human GPR174. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Bat, Bovine, Pig (89%) Marmoset, Dog, Panda, Horse, Rabbit (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from 3rd cytoplasmic domain of human GPR174. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Bat, Bovine, Pig (89%), Marmoset, Dog, Panda, Horse, Rabbit (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GPR174

Target: GPR174 (G Protein-Coupled Receptor 174 (GPR174))

Alternative Name: GPR174

Background: Name/Gene ID: GPR174
Subfamily: Orphan-A
Family: GPCR

Synonyms: GPR174, FKSG79, Jeg18, Purinergic receptor fksg79, G protein-coupled receptor 174

Gene ID: 84636