GPR77 antibody (N-Term)

Catalog No. ABIN213474

The Rabbit Polyclonal anti-GPR77 antibody (ABIN213474) specifically detects GPR77 in ELISA, IHC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for GPR77 antibody (N-Term) (ABIN213474)

Target: GPR77 (G Protein-Coupled Receptor 77 (GPR77))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR77 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GPR77 Antibody

Binding Specificity: N-Term

Specificity: Human C5L2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human (100%) Gorilla, Gibbon (95%) Monkey (85%) Marmoset (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 20 amino acid peptide from N-terminal extracellular domain of human C5L2. Percent identity with other species by BLAST analysis: Human (100%), Gorilla, Gibbon (95%), Monkey (85%), Marmoset (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GPR77

Target: GPR77 (G Protein-Coupled Receptor 77 (GPR77))

Alternative Name: C5AR2 / GPR77 / C5L2

Background: Name/Gene ID: C5AR2
Subfamily: Anaphylatoxin
Family: GPCR

Synonyms: C5AR2, G-protein coupled receptor 77, GPF77, GPR77, C5L2, G protein-coupled receptor 77

Gene ID: 27202