CHRM3 antibody (Cytoplasmic Domain)

Catalog No. ABIN213499

This Rabbit Polyclonal antibody specifically detects CHRM3 in ELISA, IHC and IHC (p). It exhibits reactivity toward Human, Rat, Mouse, Monkey, Horse, Sheep, Rabbit, Pig, Hamster, Dog, Cow, Bat and Guinea Pig.

Quick Overview for CHRM3 antibody (Cytoplasmic Domain) (ABIN213499)

Target: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Reactivity: Human, Rat, Mouse, Monkey, Horse, Sheep, Rabbit, Pig, Hamster, Dog, Cow, Bat, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRM3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CHRM3 Antibody

Binding Specificity: Cytoplasmic Domain

Brand: IHC-plus™

Specificity: Human CHRM3 / M3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Dog, Bat, Bovine, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Guinea pig (100%) Turkey, Chicken (90%) Lizard (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human CHRM3 / M3. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Dog, Bat, Bovine, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Guinea pig (100%), Turkey, Chicken (90%), Lizard (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CHRM3

Target: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Alternative Name: CHRM3 / M3

Background: Name/Gene ID: CHRM3
Subfamily: Acetylcholine
Family: GPCR

Synonyms: CHRM3, M3, Muscarinic receptor M3, Muscarinic M3 receptor, EGBRS, HM3, M3 muscarinic receptor

Gene ID: 1131

Pathways: Synaptic Membrane