FFAR2 antibody (Extracellular Domain)

Catalog No. ABIN213512

Product Details anti-FFAR2 Antibody

Target: FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FFAR2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GPR43. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except FFAR3 (47 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Monkey (94%) Mouse, Rat, Dog, Panda, Rabbit, Pig (88%) Horse, Opossum (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 17 amino acid peptide from 2nd extracellular domain of human GPR43. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Monkey (94%), Mouse, Rat, Dog, Panda, Rabbit, Pig (88%), Horse, Opossum (82%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 - 5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 35 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FFAR2

Target: FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))

Alternative Name: FFAR2 / GPR43 (FFAR2 Products)

Synonyms: FFA2R antibody, GPR43 antibody, Gpr43 antibody, GPCR43 antibody, free fatty acid receptor 2 antibody, FFAR2 antibody, Ffar2 antibody

Background: Name/Gene ID: FFAR2
Subfamily: Carboxylic Acid
Family: GPCR

Synonyms: FFAR2, FFA2R, Free fatty acid receptor 2, G protein-coupled receptor 43, G-protein coupled receptor 43, LSSIG, GPR43, Gpcr43

Gene ID: 2867